HPLC Grade water, 20Ltr

HPLC Grade water, 20Ltr

HPLC Grade Water (20L) is high-purity laboratory water specifically designed for High-Performance Liquid Chromatography (HPLC), Liquid Chromatography-Mass Spectrometry (LC-MS), and other analytical techniques that require minimal interference from contaminants. HPLC Grade water, 20Ltr undergoes advanced purification processes, including reverse osmosis, deionization, ultrafiltration, and UV treatment, to ensure the highest purity standards.

This 20-liter container provides a cost-effective, bulk solution for laboratories needing large volumes of ultra-pure water for chromatography, pharmaceutical research, and sample preparation.

Key Features and Specifications

• Ultra-High Purity: Meets or exceeds HPLC and LC-MS water specifications.
• Low Total Organic Carbon (TOC): Typically <10 ppb, minimizing background interference.
• Low Conductivity: Ensures ionic purity, typically <0.1 µS/cm.
• Particle-Free: Filtered to 0.2 µm to remove particulates and prevent column clogging.
• pH Range: 6.8 – 7.2, making it stable for analytical applications.
• Non-Leaching Packaging: Stored in a 20L chemically-resistant container to maintain purity.
• Sterile and Endotoxin-Free: Ensures reliable results in pharmaceutical and clinical research.
• No Additives or Preservatives: Free from stabilizers, ensuring compatibility with sensitive methods.

Applications of HPLC Grade Water

1. High-Performance Liquid Chromatography (HPLC)

• Used as a mobile phase component for accurate and reproducible chromatographic separations.
• Ensures low baseline noise and prevents unwanted interactions with analytes.
• Reduces peak broadening, improving resolution and detection sensitivity.

2. Liquid Chromatography-Mass Spectrometry (LC-MS)

• Essential for sample preparation, dilution, and instrument rinsing.
• Ensures minimal ion suppression and interference, allowing precise mass spectrometry analysis.

3. Pharmaceutical and Biotechnological Research

• Used in the preparation of buffers, standards, and reagents for drug development.
• Complies with stringent pharmaceutical regulatory standards for laboratory testing.

4. Environmental and Food Testing

• Required for trace-level contaminant analysis, including pesticide and heavy metal detection.
• Ensures high accuracy and reproducibility in environmental and food safety studies.

5. Clinical and Biomedical Research

• Used in clinical assays, diagnostic applications, and molecular biology experiments.
• Guarantees contaminant-free results in highly sensitive testing environments.

Purification Process

HPLC Grade Water undergoes a rigorous multi-step purification process to remove ionic, organic, and microbial contaminants:

1. Reverse Osmosis (RO) – Eliminates dissolved solids and large contaminants.
2. Deionization (DI) – Removes ionic impurities, ensuring ultra-low conductivity.
3. Ultrafiltration – Eliminates particulates and microbial contaminants.
4. UV Oxidation – Reduces Total Organic Carbon (TOC) to prevent background interference.
5. Final 0.2 µm Filtration – Removes any remaining particulates for sterility.

Each batch undergoes strict quality control testing to meet HPLC-grade specifications before packaging.

Storage and Handling Guidelines

• Storage Conditions:

  • Keep in a cool, dry place, away from direct sunlight and heat sources.
  • Store in a clean, dedicated area to prevent contamination.
  • Avoid exposure to volatile chemicals, as water can absorb airborne contaminants.

• Shelf Life:

  • Typically 12–24 months under proper storage conditions.
  • Once opened, use within the recommended period to maintain purity.

• Handling Precautions:

  • Use sterile labware to avoid introducing contaminants.
  • Do not expose to dust, plasticizers, or organic solvents.

Comparison with Other Laboratory Water Grades

Conclusion

HPLC Grade Water (20L) is an essential laboratory reagent for high-precision analytical techniques, including HPLC, LC-MS, pharmaceutical research, and environmental testing. Its ultra-low TOC, ionic purity, and stringent quality standards make it the ideal choice for laboratories that require reliable and interference-free results. With secure packaging and rigorous purification processes, it guarantees accuracy, consistency, and high sensitivity in all analytical applications.

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